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Optical control of brain functioning with optogenetics and wave front shaping

Scientific and technical environment of the training course

Institut de la vision - UMR 7210

COURSE DIRECTORS

Valentina EMILIANI

Senior researcher

UMR 7210

Eirini PAPAGIAKOUMOU

Researcher

UMR 8250

LOCATION

PARIS (75)

ORGANISATION

5 days
Training course in english
From 7 to 20 attendees

TRAINING FEES

2500 Euros

AT THE END OF THE TRAINING COURSE

Satisfaction survey from trainees
A certificate of attendance is delivered.

COURSE DATE

18269 : from monday 08/10/2018 to friday 12/10/2018

January February March April
May June July August
Sept. Oct.
18269
Nov. Dec.

OBJECTIVES

- Be able to chose the most suitable combination of actuator, illumination methods and targeting strategy for each specific application
- Learn the skills and technologies required to design and build up an optical system for optical control of neuronal circuits

AUDIENCE

Neurophysiologists, physicists (researchers, phD students or engineers)

PRE-REQUIREMENT

Attendees should have a strong background in neurosciences, in cell biology or in optical microscopy. To adapt the programme for the trainees' expectations, we invite the attendees to download and fill out the survey from our web site.

TRAINING PROGRAMME

Lectures
- Introduction: optogenetics and uncaging
- Opsin photo-cycle modeling
- Wave front shaping and liquid crystal matrix
- Computer generated holography
- Generalized phase contrast
- Temporal focusing
- Three dimensional light patterning and temporal focusing
- Two-photon optogenetics: scanning, spiral scanning, parallel illumination: examples and comparison among the different approaches
- How to build up an holographic optical set-up
- All-optical interrogation of brain circuits
- Patterned voltage and calcium imaging
- Viral vector design
- Optogenetics and vision; optogenetics and audition
Practical courses (in small rotating groups of 7 participants max with 2 trainers by group)
- How to build up an holographic optical set up (optical design and software)
- In vitro 2P patterned photostimulation
- In vitro 2P functional imaging and patterned photostimulation
- Projects from applicants (practical): proposition of projects by the participants and discussion on feasibility

Lectures (19h), workshops (12h) and presentations of projects (8h)
Detailed program available HERE

SPEAKERS

V. Emiliani, E. Papagiakoumou, N. Accanto, E. Ronzitti, D. Dalkara, C. Wyart, D. Tanese, D. di Gregorio, G. Dugué, S. Picaud (researchers), D. Oron (researcher, Weitzmann Institute) and P. Hagemman (PR, Institut for experimental biophysics, Berlin)

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