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Epigenetics: DNA methylation and bisulfite treatment

Scientific and technical environment of the training course

Laboratory for interaction of hosts pathogenes and the environment - UMR 5244

COURSE DIRECTOR

Christoph GRUNAU

Professor

UMR 5244

LOCATION

PERPIGNAN (66)

ORGANISATION

3 days (24h)
Training course in english
From 3 to 15 attendees
1 trainers for each hands-on training

TRAINING FEES

1500 Euros

AT THE END OF THE TRAINING COURSE

Satisfaction survey from trainees
A certificate of attendance is delivered.

COURSE DATE

18298 : from monday 02/07/2018 to wednesday 04/07/2018

January February March April
May June July
18298
August
Sept. Oct. Nov. Dec.

OBJECTIVES

- Learn about the bases of epigenetics and its difference with genetics
- Understand what DNA methylation is and how it can be analysed
- Become independent in designing, performing and analysing bisulfite based DNA methylation studies

PUBLICS

Researchers, engineers / analysts, breeders
To adapt the programme for the trainees' expectations, we invite the attendees to download and fill out the survey from our web site.

PRE-REQUIREMENT

Attendees should have the basic knowledge in genetics and molecular biology to understand the training content.

TRAINING PROGRAMME

Epigenetics is one of the hottest topics in molecular biology and the sciences of hereditary (formerly known as "genetics"). One of the bearers of epigenetic information that has entered the lab routine is DNA methylation. The objective of this training is to make the participants autonomous in the use of DNA methylation mapping approaches that is based on bisulphite genome sequencing, a technique that allows for single-base and single molecule DNA methylation mapping. Participants will learn about advantages and limitations of the technique from sample preparation to EWAS. Depending on their background and objectives, participants can choose between wet-bench or dry-bench.

- Introduction and round table: case studies / biological question (provided by participants)
Hands-on training. Attendees are invited to choose between:
- Experimental session (wet bench):
. bisulfite treatment
. generation of whole-genome sequencing / reduced restriction bisulfite sequencing libraries
or
- Bioinformatics session (dry bench):
. methylation call
. identification of differentially methylated regions

2 hours of theoretical training per day (round table and lecture), rest of the time (18h) hands-on training (wet-bench or dry-bench)

EQUIPMENT

Standard molecular biology equipment (pipettes, centrifuges, dry-bath, PCR machine, agarose electrophoresis etc.), personal computer with access to mainframe server

SPEAKERS

C. Grunau and S. Maury (professors)

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