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Quantitative proteomics: different approaches

Scientific and technical environment of the training course

Institut Jacques Monod - UMR 7592

COURSE DIRECTORS

Jean-Michel CAMADRO

Senior researcher

UMR 7592

Thibault LEGER

Engineer

UMR 7592

LOCATION

PARIS (75)

ORGANISATION

4 days
From 5 to 8 attendees
Training course in English
Practical sessions in subgroups of 2 trainees with one speaker per subgroup

TRAINING FEES

2000 Euros

AT THE END OF THE TRAINING COURSE

Satisfaction survey from trainees
A certificate of training is delivered.

COURSE DATE

19137 : from tuesday 26/03/2019 to friday 29/03/2019

January February March
19137
April
May June July August
Sept. Oct. Nov. Dec.

OBJECTIVES

- Define the scope of quantitative approaches in proteomics
- Be aware of the different quantification strategies
- Be able to implement an experimental approach, from sample preparation to critical analysis of the data obtained

AUDIENCE

Technicians, engineers, researchers

PRE-REQUIREMENT

A first experience in proteomic analysis by mass spectrometry

For example, to have taken the course « Proteins characterization by mass spectrometry » (Ref. 19134, this catalog) or equivalent level

TRAINING PROGRAMME

Lectures (30 %)
- Global approaches:
. label-free quantification (DDA, DIA, pSMART)
. metabolic labeling: SILAC, dynamic SILAC, pulsed-SILAC, other metabolic labeling strategies
. chemical labeling: iTRAQ, TMT and derivatives, other labeling
- Targeted approaches: SRM / MRM, PRM (relative or absolute)
Practical training (50 %)
- Sample preparation for TMT labeling and label-free quantification
- LC-MS/MS acquisitions on the instruments (Orbitrap mass spectrometers)
Trainees can bring two samples for educational purposes (contact trainers for practical details)
Data analyses workshops (20 %)
- Presentation of the main data analysis software in quantitative proteomics
- Establishment of the analysis procedures according to the type of quantification desired (relative or absolute)
- Evaluation by trainees of different software solutions

EQUIPMENT

Mass spectrometers: Orbitrap Fusion Tribrid, Orbitrap Q-Exactive Plus, Orbitrap-LTQ-Velos ETD, MALDI TOF/TOF 4800+
Chromatography : nano-LC-Easy 1000, RSLC U3000 Dionex
The laboratory has autonomous computing resources (Alineos Cluster) and dedicated software suites (MASCOT Site License, Peaks, Progenesis Qi, Byonic, Pinnacle, Proteome Discoverer, Skyline Open Source License) which can be used depending on the needs during the training session.

SPEAKERS

J.-M. Camadro (researcher), T. Léger, C. Garcia, L. Collomb (engineers) et G. Lelandais (professor)

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