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Epitranscriptomics: mapping and analysis of RNA modifications by Next-Generation Sequencing

Scientific and technical environment of the training course

UMS Ingénierie - Biologie - Santé Lorraine - UMS 2008

COURSE DIRECTORS

Iouri MOTORINE

Professor

UMS 2008

Virginie MARCHAND

Research engineer

UMS 2008

LOCATION

NANCY (54)

ORGANISATION

3 days
Training course in English
From Tuesday (09:00 am) to Thursday (06:00 pm)
From 4 to 8 attendees
Practical course with 2 teachers for the group

TRAINING FEES

1750 Euros

AT THE END OF THE TRAINING COURSE

Satisfaction survey from trainees
A certificate of training is delivered.

COURSE DATE

19190 : from tuesday 21/05/2019 to thursday 23/05/2019

January February March April
May
19190
June July August
Sept. Oct. Nov. Dec.

OBJECTIVES

- Master the basics of epitranscriptomics
- Be able to choose appropriate methods for an epitranscriptomics project
- Acquire advanced knowledge to know how to treat RNA samples for RNA modification mapping
- Be able to generate sequencing library from RNA treated samples
- Be able to hand on Illumina technology used during the training (MiSeq Illumina)
- Acquire primary expertise in data analysis

AUDIENCE

Everyone who is interested in epitranscriptomics and has a basic knowledge of RNA molecular biology (from technician to staff scientist)

PRE-REQUIREMENT

Basic knowledge in RNA molecular biology

TRAINING PROGRAMME

Epitranscriptomics is an emerging topic that is becoming very « hot » since changes in RNA modifications have been recognized as being the causes of several human diseases. The objective of this training is to give to the participants all the necessary tools to become autonomous to perform their own projects.

Theoretical part (6 h)

- Basic notions on RNA modifications : occurrence, functions, detection methods
- Methods to identify and analyze RNA modifications:
. Antibody enrichment techniques (m6A, m1A...)
. Reverse Transcription-based techniques (m1A, pseudouridine...)
. Specific chemical treatment coupled to NGS (m5C, 2'-O-methylation...)
- Introduction to bioinformatics for biologists

Practical part (wet lab) (18 h)
- RNA treatment and library preparation for two types of RNA modifications
- Quantification and quality control of the prepared libraries
- NGS sequencing on Miseq (Illumina)
- Analysis of data obtained during the training course

EQUIPMENT

Thermocycler SC8800 Agilent, Qubit ThermoFischer, Bioanalyzer 2100 Agilent, MiSeq Illumina

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