- Acquire primary expertise in mouse development
- Be able to design primary phenotyping experiment on mouse embryos
- Learn about all the primary phenotyping analysis on mouse embryos
- Be able to dissect post-implantated embryos
- Be able to determine the window of lethality in utero
- Be able to evaluate embryos viability at neonatal stage
- Increase awareness of relevant technics to image embryos
Opened to graduate students (PhD), post-doctoral scientists, researchers, and engineers
Attendees are invited to download and fill out the survey HERE to adapt the programme to the trainees' expectations.
Pre-requirement: attendees should have the basic knowledge in mouse physiology and should be able to handle mice. Mandatory respect quarantine, meaning no contact with any rodents (rodents and exotic pets included) 3 days before accessing the animal facilities. You will receive a form to sign upon your registration to the training.
This training is aimed at providing a theoretical and practical background knowledge destined to researchers and engineers that are willing to acquire primary expertise in mouse development. The topics will cover dissection of post-implantated embryos, histology, whole mount Lac Z staining and confocal imaging of whole embryos.
Courses (In the mornings)
- Mouse embryology: introduction to mouse embryonic development, determining the window of lethality of mutant mice in utero
- Phenotyping of mouse embryos: classical histology (fixation, embedding, orientation, sectioning and staining), immunohistochemistry and in situ hybridization, imaging technics (HREM, MicroCT, OPT), high frequency echography to visualize and quantify embryonic development
- Specific technics in mouse embryology: the in-vitro culture of mouse embryos / drug testing, confocal macroscopy - applications in embryology
Workshops (In the afternoons)
- Collection and fixation of mouse embryos and placentas at E10.5 (mid gestation stage)
- Observation of Whole mount Lac Z E10.5 stained embryos (Step1)
- Observation of embryos by echography
- Observation of embryos by HREM, OPT
- Collection of mouse embryos at E8.5 (early embryonic stage)
- Collection of mouse fetuses and placentas at E18.5 (just before birth)
- Observation of embryos at the confocal macroscope
- Visualization of histological sections at different stages of development. Example of abnormalities
O. Wending, H. Jacobs, G. Bou About (PhDs, managers of the Phenotyping platforms at PHENOMIN-ICS),
Y. Lutz (PhD, research engineer), M. Teletin (PhD, researcher at IGBMC)
This training is organized by PHENOMIN-ICS, the French National Infrastructure in Mouse Phenogenomics, in collaboration with CELPHEDIA Networks and Infrastructure.