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Embryologie de la souris : formation théorique et pratique

RESPONSABLES

Yann HERAULT

Directeur de recherche

UMR 7104

Olivia WENDLING

Research engineer

UMR 7104

LIEU

ILLKIRCH (67)

ORGANISATION

3 jours
Formation en français
Quarantaine de 3 jours obligatoire pour l'accès à l'animalerie
De 6 à 8 stagiaires
Date limite d'inscription 1 mois et demi avant le début de la formation

COÛT PÉDAGOGIQUE

1700 Euros

A L'ISSUE DE LA FORMATION

Evaluation de la formation par les stagiaires
Envoi d'une attestation de formation

DATE DU STAGE

20373 : du mardi 24/11/2020 au jeudi 26/11/2020

Janvier Février Mars Avril
Mai Juin Juillet Août
Sept. Oct. Nov.
20373
Déc.
OBJECTIFS
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Acquire primary expertise in mouse development
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Be able to design primary phenotyping experiment on mouse embryos
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Learn about all the primary phenotyping analysis on mouse embryos
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Be able to dissect post-implantated embryos
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Be able to determine the window of lethality in utero
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Be able to evaluate embryos viability at neonatal stage
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Increase awareness of relevant technics to image embryos

PUBLICS
Opened to graduate students (PhD), post-doctoral scientists, researchers, and engineers
Attendees are invited to download and fill out the survey HERE to adapt the programme to the trainees' expectations.

Pre-requirement: attendees should have the basic knowledge in mouse physiology and should be able to handle mice. Mandatory respect quarantine, meaning no contact with any rodents (rodents and exotic pets included) 3 days before accessing the animal facilities. You will receive a form to sign upon your registration to the training.
PROGRAMME
This training is aimed at providing a theoretical and practical background knowledge destined to researchers and engineers that are willing to acquire primary expertise in mouse development. The topics will cover dissection of post-implantated embryos, histology, whole mount Lac Z staining and confocal imaging of whole embryos.
Courses (in the mornings)
- Mouse embryology: introduction to mouse embryonic development, determining the window of lethality of mutant mice in utero
- Phenotyping of mouse embryos: classical histology (fixation, embedding, orientation, sectioning and staining), immunohistochemistry and in situ hybridization, imaging technics (HREM, MicroCT, OPT), high frequency echography to visualize and quantify embryonic development
- Specific technics in mouse embryology: the in vitro culture of mouse embryos / drug testing, confocal macroscopy - applications in embryology
Workshops (in the afternoons)
- Collection and fixation of mouse embryos and placentas at E10.5 (mid gestation stage)
- Observation of whole mount Lac Z E10.5 stained embryos (Step1)
- Observation of embryos by echography
- Observation of embryos by HREM, OPT
- Collection of mouse embryos at E8.5 (early embryonic stage)
- Collection of mouse fetuses and placentas at E18.5 (just before birth)
- Observation of embryos under a confocal macroscope
- Visualization of histological sections at different stages of development. Example of abnormalities
INTERVENANTS
O. Wending, H. Jacobs, G. Bou About (PhDs, managers of the Phenotyping platforms),
Y. Lutz (PhD, engineer on the Imaging platform at IGBMC), M. Teletin (PhD, researcher at IGBMC)
This training is organized by PHENOMIN-ICS, the French National Infrastructure in Mouse Phenogenomics, in collaboration with CELPHEDIA Networks and Infrastructure.
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