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Optical control of brain functioning with optogenetics and wave front shaping

Scientific and technical environment of the training course

Institut de la vision

- UMR 7210

Institut Carnot Voir et Entendre

COURSE DIRECTORS

Valentina EMILIANI

Senior researcher

UMR 7210

Eirini PAPAGIAKOUMOU

Researcher

UMR 7210

LOCATION

PARIS (75)

ORGANISATION

5 days
From 09:00 am to 06:00 pm
Training course in english
From 7 to 20 attendees

PEDAGOGICAL METHODS

Lectures (14h), workshops (14h) and presentations of projects (8h)

TRAINING FEES

2500 Euros

AT THE END OF THE TRAINING COURSE

Satisfaction survey from trainees
A certificate of training is delivered.

SESSION DATES

Information in this page are applicable for the first upcoming session. Before registering for the following sessions, download the program in case of possible minor changes

21198 : from monday 18/10/2021 to friday 22/10/2021

22206 : from monday 17/10/2022 to wednesday 26/10/2022

2021
January February March April
May June July August
Sept Oct
21198
Nov Déc
2022
January February March April
May June July August
Sept Oct
22206
Nov Déc
OBJECTIVES
-

Be able to choose the most suitable combination of actuator, illumination methods and targeting strategy for each specific application


-

Acquire the skills and technologies required to design and build up an optical system for optical control of neuronal circuits

AUDIENCE
Neurophysiologists, physicists (researchers, PhD students or engineers)
PRE-REQUIREMENT
Attendees should have a strong background in neurosciences, in cell biology or in optical microscopy. To adapt the programme to trainees' expectations, we invite the attendees to download and fill out the survey HERE .
TRAINING PROGRAMME
Lectures
- Introduction to optogenetics
- Opsin photo-cycle modeling
- Wave front shaping and liquid crystal matrix
- Computer generated holography
- Generalized phase contrast
- Temporal focusing
- Three dimensional light patterning and temporal focusing
- Two-photon optogenetics: scanning, spiral scanning, parallel illumination: examples and comparison among the different approaches
- How to build up an holographic optical set-up
- All-optical interrogation of brain circuits
- Patterned voltage and calcium imaging
- Viral vector design
- In depth patterned optogenetics
Practical courses (in small rotating groups of 7 attendees max with 2 trainers by group)
- How to build up an holographic optical set-up (optical design and software)
- In vitro and in vivo all-optical manipulation of neuronal circuits
- Projects from applicants (practical): proposition of projects by the attendees and discussion on their feasibility

Detailed programme available HERE.
SPEAKERS
V. Emiliani, E. Papagiakoumou, N. Accanto, E. Ronzitti, D. Dalkara, D. Tanese (researchers, IDV, Paris), C. Wyart (researcher, ICM, Paris), D. Oron, O. Yizhar (researchers, Weizmann Institute), P. Hagemman (PR, Institute for experimental biophysics, Berlin) and M. McLean Bolton (Max Planck Florida Institute for Neuroscience, Florida US)
Avis des stagiaires

"Perfect course, great coordination and organization, extremely helpful lab members" Sakir Kaan C., academic research

"This course exceed my expections in terms of training quality, quality of speakers / content, and the opportunity to meet coll new scientits !" Rosana M., academic research